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Opinion Volume 10 Issue 1
Genomic data: CDR1, CDR2, CDR3 of the invertebrate primitive antibody- new reply to evolutionnary immunologists: Part III
Michel Leclerc
Immunology of Invertebrates, Orléans University, France
Correspondence: Michel Leclerc, Immunology of Invertebrates, Div: Biochem/Biology, Orléans University, 556 rue Isabelle Romée, 45640 Sandillon, France
Received: June 30, 2025 | Published: July 18, 2025
Citation: Leclerc M. Genomic data: CDR1, CDR2, CDR3 of the invertebrate primitive antibody- new reply to evolutionnary immunologists: Part III. J Stem Cell Res Ther. 2025;10(1):142-143. DOI: 10.15406/jsrt.2025.10.00197
Abstract
Sea star Genomic data allows us, in a first time to discover « the sea star IGKappa gene and in a second time, the CDR1, CDR2, CDR3. Determining regions of the invertebrate primitive antibody. This work asserts the notion of IPA in invertebrates.
Keywords: IPA, CDR1, CDR2, CDR3, determining regions
Introduction
This work was performed with IMGT Montpellier (France).1-3
Results
They were obtained with IMGT V domain, concerning CDR are repertoried in following tables (comparisons with Pongo abelii.
IMGT/DomainGapAlign version: 4.10.3 (2021-12-06): Figure & 2
>seastar|IGKV1-5*01|33.3|||Pongo abelii
.....EQPSDTSALQGSTVVLHCSVEQYI.....NTTAIVWWSRDSVISHNKDL.KL.......SSLNTDQL.QRYSISGDASRGEFNLKIVNFTATDAASYRCQ….
Figure 1 Closest reference gene and alleles from the IMGT V domain.
Figure 2 Alignment with the closest gene and allele from the IMGT V domain.
Discussion
For the second time, the antigen HRP is recognized by the sea star antibody. The first time was situated in 2014, after the isolation of sea star IGKappa gene(affinity with the enzyme HRP) and the second one, appears in 2024, with ELISA which is more specific in terms of specificity.2 conjugates (L Protein and streptavidin associated respectively with HRP bind the antigen HRP)
At last complementary determining regions were discovered by the use of IMGT V domain. The conserved amino-acid positions 23 41 89 104 were found in the sea star sequence. Our molecule appears to have an IGAA sequence. It aligns with the mammal: Pongo abilii
IGKV1-5 and the percentage of alignments was 33 %: a similarity to an IGKV gene when it comes to conserved amino-acids
Conclusion
We mean, you’ll understand (it’s not a problem of semantic but a question of (evidences) we have to speak not of IG LIKE when compared to the sea star Anti-HRP protein but of a sort of Invertebrate Primitive Antibody.4,5
Moreover CDR1 and CDR2 have been identified. At our knowledge, no heavy chain was found in our molecule but just light chain (Kappa one).6,7 We have from now, to compose with such a model of IPA. And CDR regions. Last observations made by the mean of modelization in 3D seem to show it exists also a CDR3 determining region.
Acknowledgments
None.
Conflicts of interest
The author declares that there is no conflicts of interest.
References
- Polonelli L, Ponton J, Elguezabal N, et al. Antibody complementarity-determining regions (CDRs) can display differential antimicrobial, antiviral and antitumor activities. PLoS One. 2008;3(6):e2371.
- Ehrenmann F, Kaas Q, Lefranc MP. IMGT/3Dstructure-DB and IMGT/DomainGapAlign: a database and a tool for immunoglobulins or antibodies, T cell receptors, MHC, IgSF and MhcSF. Nucleic Acids Res. 2010 Jan;38(Database issue):D301–307.
- Ehrenmann F, Lefranc MP. IMGT/DomainGapAlign: IMGT standardized analysis of amino acid sequences of variable, constant, and groove domains (IG, TR, MH, IgSF, MhSF). Cold Spring Harb Protoc. 2011;2011(6):737–749.
- Leclerc M. Determination of the CDR (CDR1, CDR2) complementary determining region invertebrate primitive antibody from sea star. Ann Immunol Immunother. 2024;6(4):000183.
- Brillouet C, Leclerc M, Binaghi RA, et al. Specific immune response in the sea star Asterias rubens: production of "antibody-like" factors. Cell Immunol. 1984;84(1):138–144.
- Vincent N, Osteras M, Otten P, et al. A new gene in rubens: a sea star Ig kappa gene. Meta Gene. 2014;2:320.
- Leclerc M, Baerlocher L, et al. Mapping on sea-star MHC genes in invertebrates. Eur J Biol Biotechnol. 2021;2(2):60.
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