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Research Article Volume 3 Issue 3
Antimicrobial susceptibility pattern of enterococci isolated from clinical specimens at mymensingh medical college hospital, Mymensingh, Bangladesh
Farzana Boby, Salma Ahmed, Shyamal Kumar Paul, Syeda Anjuman Nasreen, Najia Haque, Sangjucta Roy, Farid Ahamed, Shabiha Monwar, Nobumichi Kobayashi, Muhammad Akram Hossain
Department of Microbiology, Mymensingh Medical College, Bangladesh
Department of Microbiology, Jamalpur Medical College, Bangladesh
Department of Microbiology, Marks Medical College, Dhaka, Bangladesh
Department of Hygiene, Sapporo Medical University, Japan
Received: October 19, 2016 | Published: December 12, 2016
Citation: Boby F, Ahmed S, Paul SK, et al. Antimicrobial susceptibility pattern of enterococci isolated from clinical specimens at mymensingh medical college hospital, Mymensingh, Bangladesh. J Bacteriol Mycol Open Access. 2016;3(3):241-244 DOI: 10.15406/jbmoa.2016.03.00061
Abstract
Enterococci are part of the normal intestinal flora of humans and animals but have also emerged as important pathogens responsible for serious infections in hospital and community acquired infections. The aim of this study was to investigate the prevalence of Enterococcus with their antimicrobialsusceptibility pattern from patients ofMymensingh Medical College Hospital, Bangladesh during the period from July 2015 to June 2016.Samples were collected and identification of enterococcus was done by standard microbiological procedure and biochemical tests.Multiplex PCR was performed by using species specific primers for Enterococcus faecalis (E. faecalis) and Enterococcus faecium (E. faecium),vanA, vanB, vanC1, vanC2/C3 genes for vancomycin resistance.A total of 105 enterococci were isolated from 1201 different clinical specimens (from urine (931), pus (175) and wound swab (95) respectively) from July, 2015 to June, 2016.The prevalence of enterococci was 12.34% in urine, 17.17% in pus and 1.58% in wound samples. Out of 105 enterococci,80 (77.14%) were Enterococcus faecalis,1 (0.95%) was E. gallinaramand 24 (22.86%) were other species. All the species were highly resistant to aminoglycosides (Gentamicin) (75.24%) and ciprofloxacin (73.33%). Regarding antibiotic sensitivity, all the enterococci were (100%) sensitive to linezolidand vancomycin followed by nitrofurantoin 93 (88.57%) and imipenem 89 (84.76%). No vancomycin resistant enterococci were identified by disk-diffusion method. But by PCR, vanB was found in 1/80 (1.25%) of the E. faecalis isolates and 01 (100%) of the E. gallinarum.vanC1 was detected in 1 (100%) ofE. gallinarum isolates. The vanA and vanC2/C3 gene was not found in any isolates. For the first time, one isolate of E. gallinarum has been found harboringthe vanB gene in our hospital.The presence of multidrug resistant enterococci should be considered as danger alarm for serious enterococcal infections and further study in large scale is needed.
Keywords: Bangladesh; Enterococci; VanA; VanB; VanC1; VanC2/C3; Vancomycin resistant enterococci; Antibiotic sensitivity
©2016 Boby, et al. This is an open access article distributed under the terms of the, which permits unrestricted use, distribution, and build upon your work non-commercially.